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The determination of early apoptosis was done at 24 h by aspirating the MM cells away from the BMSC and using Annexin V assay with flow cytometry and cytotoxicity at 96 h using DIMSCAN assay as previously described

Furthermore, inactivation of these proteins leads to inhibition of both NAMPT signaling and a CSC-like phenotype

Nutrients 14 , 2063 (2022)

For instance, lactate metabolism has been studied as a modulatory agent to induce specific signaling transduction, and lactate enters the cells through MCTs and engages with GPR81 receptors in an autocrine manner, thereby reducing cAMP generation and modulating the insulin-induced PI3K-AKT pathway [39,40,41]

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Future comprehensive clinical care depends on identifying new therapeutic targets and adopting a multi-therapeutic approach