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Impairments in neuronal survival mediated by HMGB1 are believed to be closely associated with the glycolytic pathway, as evidenced by the mitigation of these adverse effects through treatment with the glycolysis inhibitor 2-DG [194]

Also, a decrease in the level of choline, the precursor of the neurotransmitter ACh and the agonist for nicotinic-cholinergic receptor (nAChR), was determined in individuals diagnosed with ASD (Friedman et al., 2006), with the severity of ASD related to the decreased level of cytosolic ACh (Sokol et al., 2002)

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A 2021 review performed by the nonprofit organization Association for Science in Autism Treatment found that the Brain Balance approach lacks sufficient empirical evidence and should therefore be considered a pseudoscientific treatment. The current program is based on contemporary developmental neuroscience and supported by a modern evidence base developed independently of any former contributors, a Brain Balance representative told The Transmitter