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Non-invasive sampling methods such as liquid biopsy, as described above, are also rapidly progressing as a supplement or alternative to solid biopsy

All real-time PCRs were performed three times and all changes in gene expression were reported as multiples of increases relative to the control while GAPDH was used as an internal control and relative gene expression between the experimental groups were determined using MxPro software

Role of subclinical gut inflammation in the pathogenesis of spondyloarthritis