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The supernatant was neutralized with saturated potassium carbonate and incubated for 15 min at room temperature and then centrifuged at 13,000 rpm for 10 min

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RanGTP and PlexinB1 partially colocalise in the cytoplasm and plasma membrane of Cos7 cells co-transfected with RanQ69L-cherry and cytoPlexinB1-myc (Fig