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Western blotting Cell lysates were collected in RIPA buffer (150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulphate)and Western blotting was performed as previously described 49

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[109] described an increased apoptosis of alveolar epithelial and ECs in lung tissue from patients with emphysema

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