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A quick way to decide: start with the solvent on the certificate of analysis, fall back to bacteriostatic water for a standard sequence, and only reach for acid or base when a peptide visibly resists water

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Supplementary Data 5 (download XLSX Statistical source data

Buffer B: 0.08% formic acid in 80:20 acetonitrile: water, 0.7 min at 1.8 L/min from 1% to 4% B, 0.3 min at 1.8 L/min from 4% to 8% B, 6.7 min at 1.8 L/min from 8 to 22.5% B, 3.7 min at 1.8 L/min from 22.5 to 35% B, 0.4 min at 2.5 L/min from 35 to 55% B)

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Nevertheless, how alcohol consumption increases breast cancer risk is still subject to discussion (151)