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Protein purification confirmation Isolated AcrIIa4 protein was run on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue staining to confirm protein size (Supplemental Figure 3)

Magnesium is one of those foundational minerals that touches so many systems at once, says Carmen Fong, MD, chief medical officer at Bummed and a colorectal surgeon