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[3] Biological activity: The glutathione redox cycle Glutathione exists in two interconvertible forms, reduced glutathione (GSH) and oxidized glutathione (GSSG)

Aerobic energy metabolism, or oxidative phosphorylation, is a critical metabolic pathway within cells to provide the energy necessary to complete our daily tasks

For example, stimulation of viral replication by inflammatory cytokines is optimal at decreased glutathione concentrations

To avoid metHb accumulation, reducing pathways mediated by cytochrome b5 or flavin coupled with NADH- or NADPH-dependent metHb reductases (MHbR), respectively, keep the level of metHb in erythrocytes at less than 1% of the total Hb under normal conditions (48)

Glutathione has also been studied for its potential to combat oxidative stress, inflammation, and mitochondrial dysfunction

After the reduction, a second alkylation step was carried out by adding 5 l of 100 mM d5-NEM