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Additionally, MDSCs and NK cells accumulated in pre-metastatic and metastatic niches support tumor cell dissemination and colonization through modulation of immune responses (Quail and Joyce, 2013)

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doi: 10.1038/bjc.1997.238 66 Di VirgilioFAdinolfiE

After 30 min incubation on ice and centrifugation at 16000 g for 20 min at 4C, the amount of protein in the precipitate was determined using the DC protein assay (Bio-Rad Laboratories) according to manufacturer's recommendations (Bulletin 1770 US/EG Rev A, Bio-Rad)