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The next day, cells were collected and washed with PBS and solubilized in a buffer containing Brij 97 (1% Brij 97, 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM EDTA and phosphatase and protease inhibitors) for 30 min with regular agitation at 4 C

(PubMed) Spagnoli A, Lucca U, Menasce G, et al

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NF-B signaling in inflammation

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Interestingly, bacteriophages have been genetically modified to express depolymerase during infection, enabling the simultaneous degradation of the bacterial cell wall and the biofilm matrix, thereby enhancing the chances of biofilm destruction (Lu and Collins, 2007)