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To test this idea, artificial microRNA (amiRNA) constructs targeting two different loci of 3 UTR of -GLUTAMYLCYSTEINE SYNTHETASE ( GSH1 , the rate-limiting enzyme required for glutathione biosynthesis) were generated (amiGSH1-1 and amiGSH1-2) and introduced into the smt15-1 mutant (Figure 3A)

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Outcome over seven years of healthy adults with and without subjective cognitive impairment