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Evolution of white matter damage in amyotrophic lateral sclerosis

In the spinal nerve ligation model, in which anatomic nerve regeneration can be easily observed, nicotine reduced anatomic measures of regeneration as well as expression of regeneration marker growth-associated protein 43 (GAP43)

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For BODIPY 493/503 staining, samples were incubated with 2 M BODIPY 493/503 (1 ml/well) for 30 min at RT

A pronounced force on the discs can herniate them, as well, because dehydrated discs cannot sustain impact and have reduced elasticity

Alvarez-Carbonell D, Ye F, Ramanath N, Dobrowolski C, Karn J