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Systemic copper metabolism Cu is an essential element in virtually all living organisms, and numerous studies have shown that Cu serves as a cofactor for a variety of key metabolic enzymes that drive a broad range of physiological processes

The enzyme activity was terminated by heating the solution at 95 C for 15 min to denature the enzyme

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These findings highlight the critical regulatory role of PrP in copper metabolism and open new avenues for exploring the therapeutic potential of PrP suppression in Wilson disease

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