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A modified mutant human NNMT (mt-hNNMT) [lacking 3 amino acid residues from the C-terminus of the NNMT protein that was not observed in crystal structure] (3ROD, PDB accession code) cloned into an IPTG-inducible plasmid pJ401 expression vector was purchased from DNA 2.0 (Menlo Park, Calif.)

Acute dosing raises whole-body energy expenditure even in mice that lack the beta-3 adrenergic receptor, which means part of the effect runs through other pathways

The carrier base matters substantially for topical GHK-Cu dosing protocols