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The following day, this medium was refreshed, with the expansion medium lacking rho kinase inhibitor but continuing with 5 nM scTS2/16

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When RPL15 is overexpressed, the number of nucleoli and the expression of nucleolar proteins increases, and the abnormal increase in nucleolar size and number caused by dysregulation of ribosome biosynthesis is a hallmark of most spontaneous cancers [47]

TABLE 1 Summary of mechanisms leading to tamoxifen resistance The Discovery of LEM4 and the Association Between Cell Cycle Regulators and Resistance to Tamoxifen As a competitive antagonist of estradiol, tamoxifen can bind to estrogen receptors in competition with estradiol and form a stable complex, which inhibits the transcription activity of the estrogen receptor and blocks breast cancer cells in G1 phase to inhibit tumor proliferation