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102 Furthermore, interdisciplinary collaborative innovation enables the exploration of modern pharmacological mechanisms of active components in traditional Chinese medicine (such as artemisinin and arsenic trioxide)

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Along with the formation of GSNO after reaction with GSH, NO can readily react with the sulfhydryl group (-SH) of protein cysteine residue to form S-nitrosocysteine that consists of a redox-mediated posttranslational modification

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For TP53-R72P overexpression experiments fully differentiated (day 7) C3H10T1/2 CrispR/Cas9 p53 KO adipocytes were detached using trypsin (0.25%)/collagenase (0.5 mg/ml), washed with PBS and resuspended in electroporation buffer R (Neon Transfection system, MKP1096, Invitrogen) to 6 10 5 cells per 10 l

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