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The effect of yiqi huoxue tongluo decoction on spinal cord microglia activation and ASK1-MKK3-p38 signal pathway in rats with diabetic neuropathic pain

PMID 8348154

doi: 10.3389/fimmu.2020.00391 Received 28 December 2019 Accepted 19 February 2020 Published 24 March 2020 Volume 11 - 2020 Edited by Jorge Matias-Guiu, Complutense University of Madrid, Spain Reviewed by Ulises Gomez-Pinedo, Instituto de Investigacin Sanitaria del Hospital Clnico San Carlos, Spain

The contact time during washing matters too

We unveiled that SP could remodel bone homeostasis in RA by simultaneously targeting SMs and osteoclasts in vitro

To remove residual reagent or precipitates, sections were washed with 50% ethanol for 23 min, followed by PBS and 0.3% Triton X-100 in PBS for 4 min