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SGLT2 inhibitors (SGLT2i) provides nephroprotective benefits in to human patients with DKD, presumably by suppressing HIF1A and stimulating HIF2A, which in turn augments erythropoiesis 44,45,46,47

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After 3 h drug exposure, cells were detached, washed twice with phosphate-buffered saline (PBS), dried at RT for 15 min and lysed in 100 L HNO 3 (69%, Rotipuran Supra, Carl Roth, Karlsruhe, Germany) for 1 h at RT