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Once the His-tagged protein is bound to the metal-resin, contaminating proteins can be washed away leaving the desired protein that may be eluted in a high imidazole concentration buffer

The UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A9, UGT2B7, and UGT2B15 belong among the main liver xenobioticconjugating enzymes, whereas UGT1A7, UGT1A8, and UGT1A10 are predominantly extrahepatic UGT forms