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Upon OD 600 1 normalization the bacteria were resuspended in TSB-chloramphenicol with 0.5mM IPTG and 250L of this mix was transferred to a sterile Eppendorf tube into which 640M 4-HNE was added

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doi: 10.1038/ncomms2852 30 KleinerD

In contrast, some flavonoids influence the cell membranes of specific bacteria (such as Staphylococcus aureus and Escherichia coli ), either by directly disrupting the lipid bilayer of the cell membrane or by modifying the permeability of the cell membrane, ultimately hindering the proliferation of these bacteria (Xie et al., 2015)

These non-viable constituents employ beneficial effects on the host as they actively modulate immune responses by that inflammation is reduced, supporting the gut barrier integrity (124)