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Cells were harvested and centrifuged at 1200 rpm for 3 min, followed by incubation with 1 L annexin V-Alexa Fluor 488 (640906, BioLegend) in 100 L annexin V binding buffer 1 (10 mM HEPES (pH 7.4), 0.14 M sodium chloride (NaCl), 2.5 mM calcium chloride (CaCl 2 ) and incubated at room temperature in the dark for 15 min

Khler ES, Sankaranarayanan S, van Ginneken CJ, van Dijk P, Vermeulen JL, Ruijter JM, Lamers WH, Bruder E (2008) The human neonatal small intestine has the potential for arginine synthesis

TREATMENT Early initiation of treatment is critical immediately following recognition of DILI

A working description of sleep as an electro physiologically and behaviourally defined state has been well-known

Malik, S., Theis, M

The molecules planar structure also enhances its interactions with biomolecules, including DNA, enzymes, and receptors, further boosting its anticancer and anti-inflammatory effects [12, 13]