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Primers were identified and removed using cutadapt (v2.7) with a maximum error rate of 0.2, reads lacking both forward and reverse primers were discarded

DPC MedLab, Los Angeles, CA, USA)

AlphaPlex is a further development that allows quantification of up to three analytes by using different acceptor beads that emit at distinct wavelengths

Moreover, the pathological mechanisms of these endothelial injuries seem to be attributed to disorders of the NO/cGMP pathway, abnormal endothelium-derived vasoactive factors, oxidative stress, etc

This assay was also carried out in primary astrocytes to determine cell viability in non-tumor cells ( Supplementary Material )