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Common limiting factors Poor microvascular supply: Tendons and ligaments may not receive enough oxygen and nutrients to sustain remodeling Chronic inflammatory signaling: Inflammation may remain dysregulated after the initial stimulus Disorganized collagen remodeling: Tissue repairs, but strength and alignment remain suboptimal Age-related regenerative decline: Older tissues respond less robustly to growth factor signaling Incomplete post-procedure support: Biology is stimulated but not supported long term PRP and prolotherapy light the match

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It is well reported that the constant activation of the NMDA and AMPA receptors modifies the homeostasis of intracellular calcium, thus modifying its function as a second messenger and major modulator of calcium-dependent enzymes, such as calmodulin and nitric oxide synthases 1 and 2 (NOS-1 and NOS-2)