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For histone analysis, cell pellets were acid-extracted overnight using Abcams histone extraction protocol in phosphate-buffered saline containing 0.5% Triton X-100, 2 mM PMSF, 0.02% NaN 3 , and 10 mM sodium butyrate and supplemented with cOmplete Protease Inhibitor Cocktail before use

These toxic metals can also disrupt redox signaling, enhance calcium influx, and activate downstream pathways like NF-B, further amplifying cytokine production and inflammation (Chen & Shi, 2002

Hypoxic state-induced DNA damage is a common phenomenon, especially during muscle overuse

Therefore, At-NEET might be a carrier protein for the assembly and transfer of NEET-type 2Fe-2S clusters (Nechushtai et al., 2012)

Were healing from within

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