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and the staff at the Biophysics Platform, Protein Structure and Function Program from The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen for performing the human serum albumin SPR binding measurements

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It does this by creating a chemical reaction within the body, rewiring the respiratory chain to output hydrogen peroxide and changing how the body uses oxygen

Following centrifugation (20,000 x g at 4 C for 10 min), supernatants were collected, and sample protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Scientific, 23225) with bovine serum albumin as a protein standard

At present, five drugs have entered phase 3 development for the treatment of NASH, but the selonsertib Phase 3 program has been recently discontinued

doi: 10.1046/j.1365-2958.1998.01062.x