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Proteins were precipitated by adding four volumes of ice-cold acetone and subsequent centrifugation (21.000 g , 20 min, 4 C)

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KOC-7C cells were provided by the Gynecological and Obstetrics Department, Kurume University and others were provided by the Gynecological and Obstetrics Department, Osaka University [9]

5-MTHF measurements in CSF were reported in 15 cases with AR-GTPCH, 83 cases with PTPSD, 63 cases with DHPRD and 3 cases with SRD

Taken together, these data demonstrated that 1D228 exerted powerful antitumor activity in vivo