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In addition, four rats exhibited hepatic necrosis inflammatory cell infiltration (severe in one rat, moderate in two rats, and mild in one rat) (Lebda et al., 2017b)

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The released inhibitor not only inhibited the influx of pyruvate into the mitochondria but also effectively blocked the tricarboxylic acid (TCA) cycle, which is fueled by glucose and lactate, thereby disrupting aerobic respiration and alleviating tumor hypoxia (Figure 6B)

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buffer B acetonitrile, 0.1% TFA) with a C18 Hypersil-ODS (5 m, 250 4.6 mm) column and a flow rate of 1 ml/min