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Significant increases in cellular GSH level were detected in cultures grown at 100 g/ml or 150 g/ml 5-FOA, but not at 50 g/ml 5-FOA (Figure S2A)

The melting temperature ( T m ) values were calculated using the LightCycler Thermal Shift Analysis software (Roche Applied Science) and Origin 8.0 software

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The deviation of the sigmoid curve fit, found after the T50 is reached, suggested the presence of a subpopulation of cells, better equipped to withstand oxidative stress, or a different distribution in the heterogeneous population as shown in Fig

In addition, an extended ZSWIM8 loop within HEAT-repeat 3 anchors the MID domain, primarily through a phenylalanine (F1261) inserted into an exposed hydrophobic cavity of the MID domain

Nozawa et al., 2008)