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Briefly, isolated crypts were centrifuged for 2 min at 68 g , resuspended in the appropriate volume of cold mouse colonic organoid growth medium (bioGenous, K2204-MC) and embedded in equal volume of Matrigel matrix (ABW, 082703) at 50250 crypts/20-30 L in a 48-well plate

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doi: 10.1007/s00299-008-0596-8 169 TanY.-J.KhooS

Mushrooms have high amounts of the ergothioneine and glutathione, both important antioxidants, said Robert Beelman, Professor Emeritus at Pennsylvania State University in the US