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DISCUSSION In this study, we successfully applied CRISPR/Cas9 technology to generate a stable genetic ndufs2 -/- 16 bp deletion zebrafish line that had 80% reduced CI enzyme activity, 96% reduced ndufs2 mRNA expression, significantly reduced lifespan (mean 10 dpf, max 11 dpf), swimming-based neuromuscular activity, and multiple gross morphological changes including linear growth defect, hepatomegaly, uninflated swim bladder, yolk retention, small intestinal size, and small eye and pupil size with an abnormal retinal ganglion cell layer

p = 0.0617) but was nonsignificant when analyzed separately in the NAC group ( r = 0.5254

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Psychiatry 27 , 20192029 (2022)