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Similarly, in a study conducted by Yan et al., antioxidant peptides GWY and QWY exhibited RMSD fluctuations of approximately 0.19 0.01 nm during 25 ns simulations, and their corresponding free energy landscapes displayed well-confined energy basins within strict boundaries (40)

2 Materials and methods 2.1 Animals Female Sprague-Dawley rats were purchased from the animal facility of the Central Animal Breeding Service Center at Army Medical University (AMU, Third Military Medical University), Chongqing, China