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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP

It is an antioxidant It inhibits inflammation It improves insulin sensitivity, which lowers the risk of diabetes It is protective to the brain, and is thought to be a potential treatment for Parkinsons and Alzheimers It regulates the balance between regeneration and apoptotic cell shedding in the intestine

Shiga S, Machida T, Yanada T, Machida M, Hirafuji M, Iizuka K (2020) The role of nitric oxide in small intestine differs between a single and a consecutive administration of methotrexate to rats

Higher bioavailability and better cellular uptake compared to standard supplements

doi: 10.2174/1389200222666210202110129 186

J., Adam, K