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Steps 29 had the following profile: 10 min of X% buffer C, a 15 min gradient from 0% to 5% buffer B, and a 95 min gradient from 15% to 100% buffer B

These findings align with previous studies that demonstrated the negative impact of applying A

For growth in L-DOPA medium, cells were inoculated in chemically-defined L-DOPA medium containing 0.1% L-asparagine, 0.1% dextrose, 3mg ml -1 KH 2 PO 4 , 0.25 mg ml - 1 MgSO 4 7H 2 O, 1 g ml - 1 thiamine, 5 ng ml - 1 biotin, and 0.2 mg ml - 1 L-3,4-dihydroxyphenylalanine (L-DOPA

M., & Hanggi, G

Frontiers in Pharmacology , 2022

It counteracts the poisonous effects of acetaldehyde