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To remove the excess of IAA completely, samples were separated under non-reducing conditions on SERVAGel TG PRiME 4-12% (SERVA, Heidelberg, Germany)

Ethical and Practical Considerations: If your study involves a larger cohort where ease of administration is paramount, and you're willing to account for potentially lower or more variable bioavailability, an oral formulation might be explored

The relative protein levels were normalized to that of -actin and quantified using ImageJ

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