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DMT-1 is expressed at the brain endothelial and functions as a high-affinity transporter for non-transferrin-bound divalent cations, exhibiting a non-selective affinity for various divalent metal ions, including Mn [15, 133]

CBS deficiency led to markedly homocysteine accumulation and severely decreased levels of cystathionine and cysteine, which was due to impaired homocysteine removal through the transsulfuration pathway [32]

Human studies have shown that as the severity of liver fibrosis progresses, the number of hepatic progenitor cells increase, mimicking the pathological processes associated with the wound healing and carcinogenic response of the liver to injury which is observed in chronic viral hepatitis and alcohol-related liver disease [91, 92]

doi: 10.1007/s43440-023-00542-9, 4

CBS proteins with a permanent C-terminal His-tag were purified using TALON (Clontech) resin, followed by anion exchange using a Hitrap Q column (Cytiva)

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