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however, further studies are needed to elucidate the role of mitochondria in chemoresistance in acute leukemia

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E.coli lysate containing an iminobiotinylated protein (~ 45 kDa) was 1: 5 diluted in Binding Buffer (50 mM (NH4)2CO3, 0.5 M NaCl, pH10.0) and then was loaded on 0.5 mL packed Streptavidin 6FF resins. After washes with the Binding Buffer, bound material was eluted in Elution Buffer (50 mM NH4Ac, 0.5 M NaCl, pH 4.0 ). The resulted resin was then incubated with SDS sample buffer at 95C for 10 minutes. Fractions were analyzed by SDS-PAGE