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The sample was loaded onto a 5-ml HiTrap SP HP column (Cytiva, 17115101) equilibrated with buffer B/150 mM NaCl

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In contrast, studies on human senior subjects show that low IGF-1 levels are associated with poor knee extensor muscle strength, slow walking speed, and difficulty with mobility tasks [6]

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[8] One such potential pathway involves the activation of phospholipase C (PLC), which researchers suggest may lead to the release of inositol triphosphate (IP3) and diacylglycerol (DAG)