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Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenase Activity in Cultured Cells

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Rio and Velez-Pardo, 2008

acidocaldarius MW00G was routinely grown at 76 C under constant shaking at 120 rpm (New Brunswick Innova 44127 incubator, Eppendorf, Hamburg, Germany) in Brocks basal salt medium 62 , supplemented with 10 g mL 1 of uracil and 10 mM, 20 mM, or 40 mM glycerol, or for comparison with 0.2% (w/v) D-xylose as sole carbon and energy source

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