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In brief, cDNA was generated using M-MLV reverse transcriptase (Thermo Fisher) from RNA samples isolated from the cultures, and then incubated with Fast SYBR Green Master Mix (Applied Biosystems)

10.1016/0006-2952(87)90130-4 Biochem

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With the discovery of a new allosteric site of KRAS (G12C), several irreversible covalently binding inhibitors of KRAS (G12C) have emerged, raising the hope of drugging KRAS