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Cultures were passaged by trypsinization (0.05% trypsin-0.53 mM EDTA) every week and were seeded at a density of 1.2 10 4 cells/cm 2 in 60 20 mm or 100 20 mm diameter culture dishes (Sarsted, Nmbrecht, Germany), 96-well or 24-well plates (Sarstedt), or 15 -slide 4-well coverslip (Ibidi USA, ThermoFisher Scientific) for subsequent experiments