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Schwartz, MD, FACS Mary E

Lysates were then sonicated, boiled, and stored at 20 C until needed

Isolation and Structure Determination of a Candidate Tetradepsipeptide Ethyl Ester ( m/z 453) and Octadepsipeptide Ethyl Ester ( m/z 837), Respectively The combined EtOH extracts obtained from cultures (100 mL) of strain F4810/72 were concentrated to about 25 mL in vacuum and, after dilution with H 2 O (1/10, v/v), aliquots (5 mL) were separated by means of preparative RP-HPLC on a PrepStar system (Varian, Darmstadt, Germany) consisting of two HPLC-pumps (Model SD-1), a two-wavelength UV detector (Prostar 325), a fraction collector (Model 701) and equipped with a 250 21.2 mm, 4 m, 90A, Jupiter Proteo column (Phenomenex) as the stationary phase

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A total of 177 natural products with greater potential for AD treatment were screened by mapping the target gene set of each natural product to the constructed network, yielding 177, 103, and 29 natural products from Most Studied Pathways, Gene-Associated Pathways, and Popular Pathways, respectively (Additional file 1: Table S7)