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Through their functional activities of post-translational modifications (ADP-ribosylation and deacetylation), or through the modulation of Ca 2+ signaling, these enzymes regulate gene transcription, cell differentiation, cell cycle progression, circadian rhythm, DNA repair, chromatin stability, cell adaptation to stress signals, and immune responses ( Extracellular NAD and Its Biological Role The eNAD concentration is in the range of 100500 nM, considerably lower than its intracellular levels (200500 M) (Figure 1), despite the presence of extracellular precursors and biosynthetic enzymes

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