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Cell debris was removed by centrifugation (20 min, 4 C, 15,000 g ), and the protein concentration of the supernatants was determined using the Pierce BCA (Thermo Fisher Scientific) with a bovine serum albumin standard (yielding an average of 5.8 g/L protein)

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Williams DS, Cash A, Hamadani L, Diemer T: Oxaloacetate supplementation increases lifespan in Caenorhabditis elegans through an AMPK/FOXO-dependent pathway

The study demonstrated that the injection of flatulin in the rat MCAO model significantly inhibited ferroptosis and reduced the size of cerebral infarcts and alleviated the neurological damage after cerebral ischemia in rats, providing new insights into the IS (Jayakumar et al., 2013)

under BH 4 -deficient conditions, superoxide is generated instead of NO, increasing the susceptibility of cells to ferroptosis[95]