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Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)

mellonella were treated with palmatine and berberine to test effects on mortality, pupation and diet ingestion

The MAPK/ERK pathway is another key signaling pathway in GC, involved in processes such as cell proliferation, differentiation, stress response, and migration

The column can often be equilibrated and eluted with lower guanidineHCl concentrations (e.g., 4 M) than those used for the actual extraction process

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