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Consistent with the model proposed by Datsi and Garg, TIL plus PD-1 strategies aim to increase both the number and functional quality of tumor-specific T cells beyond a critical threshold, rather than merely releasing inhibitory brakes on an insufficient endogenous repertoire [24]

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Our previous work in human macrophages indicates that HIV blocks the formation of the apoptosome and that in surviving cells Bim, a highly apoptotic protein, is recruited to the mitochondria without resulting in apoptosis 23 , suggesting that in HIV surviving cells, mitochondrial function is compromised

Neither option is universally "better." Many laboratories routinely use BAC water because its preservative supports repeated handling, while certain experimental protocols specifically require preservative-free sterile water