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Finally, tissue sections were mounted with Vectashield plus DAPI (4,6-diamidino-2-phenylindole) (Vector Labs) and imaged with an LSM 980 confocal microscope (Zeiss)

10.4049/jimmunol.0902566 21 GuptaT.MullinsM

It also shows how environmental factors like infections and toxins amplify this dysregulation, and how functional testing can illuminate root contributors and guide targeted interventions

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For toxicity testing performed over a 12-day experimental period, tissues were daily exposed to 3 different concentrations of each test material for 30 min or 6 h (for detailed information on each material see Table 1)