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Description

Nat Cell Biol (2015) 17(12):155668

Pawelec, G

doi: 10.1038/s41418-023-01125-0

FACS Extension Agents - Rachel Stewart, Terri Black, Susan Moore, Laura Smith, Georgeanne Cook, Jackie Ogden, Leslie Thomas, Becky Collins, Carrie Vanderver, Alshleigh Guerin, Allison Eaddy, Jamile Hawkins

166 In addition, ATF4 binds to cAMP response elements (CREs) within the promoters of LC3B and ULK1 , increasing autophagosome formation

The bound protein was eluted using 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, and 5 mM EGTA and 0.1 mg/mL Flag peptide