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On the next day, targeting plasmids (sgRNA or overexpression) were co-transfected with pMD2.G (#12259, Addgene) and psPAX2 (#12260, Addgene) plasmids at 1:1:1 molar ratio using Lipofectamine 2000 (#11668019, Thermo Fisher) at 1:2.5 ratio

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The lab's identifier and test date are published on the Certificate of Analysis for that batch, including explicit confirmation that the Cys7Cys14 disulfide bond is intact

15 mg vial reconstitution chart For the 15 mg vial, the 10 mg/mL concentration (1.5 mL water) keeps the math clean and consistent with the 10 mg vial charts above

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Immunofluorescence microscopy Preparation of immunofluorescence samples was performed as described 79