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Membranes were then incubated overnight at 4C with the following primary antibodies: NINJ1 (1:1000, Invitrogen, PA5-95755), GAPDH (1:1000, Cell Signaling, #97166), PANK1 (1:1000, Cell Signaling, #23887), xCT (1:1000, Cell Signaling, #12691), V5 tag (1:1000, Cell Signaling, #13202), and -tubulin (1:1000, Cell Signaling, #2128)

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The duration of storage may also affect the physical and chemical properties of fermented mushroom such as color change, decreased pH value, and reduced anthocyanin content (Nurerk et al., 2022)

Fluvastatin synergistically enhances the antiproliferative effect of gemcitabine in human pancreatic cancer MIAPaCa-2 cells