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Toxicol Appl Pharmacol 15(435):115853 Moore EM, Mander AG, Ames D et al (2013a) AIBL Investigators Increased risk of cognitive impairment in patients with diabetes is associated with metformin

Methods Cell viability, Apoptosis, activation and inflammatory responses of microglia, expressions and activity of Shh signaling pathway proteins were detected by Cell Counting Kit 8, Flow Cytometry, immunocytochemistry, ELISA, and Western blotting, respectively